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Reference Detail
| Reference | ||
|---|---|---|
| Author | Jing H., Yang X., Zhang J., Liu X., Zheng H., Dong G., Nian J., Feng J., Xia B., Qian Q., Li J., Zuo J. | |
| Title | Peptidyl-prolyl isomerization targets rice Aux/IAAs for proteasomal degradation during auxin signalling. | |
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Abstract: In plants, auxin signalling is initiated by the auxin-promoted interaction between the auxin receptor TIR1, an E3 ubiquitin ligase, and the Aux/IAA transcriptional repressors, which are subsequently degraded by the proteasome. Gain-of-function mutations in the highly conserved domain II of Aux/IAAs abolish the TIR1-Aux/IAA interaction and thus cause an auxin-resistant phenotype. Here we show that peptidyl-prolyl isomerization of rice OsIAA11 catalysed by LATERAL ROOTLESS2 (LRT2), a cyclophilin-type peptidyl-prolyl cis/trans isomerase, directly regulates the stability of OsIAA11. NMR spectroscopy reveals that LRT2 efficiently catalyses the cis/trans isomerization of OsIAA11. The LRT2 mutation reduces OsTIR1-OsIAA11 interaction and consequently causes the accumulation of a higher level of OsIAA11 protein. Moreover, knockdown of the OsIAA11 expression partially rescues the LRT2 mutant phenotype in lateral root development. Together, these results illustrate cyclophilin-catalysed peptidyl-prolyl isomerization promotes Aux/IAA degradation, as a mechanism regulating auxin signalling. |
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| Journal | Nat Commun | |
| Country | China | |
| Volume | 6 | |
| Pages | 7395 | |
| Year | 2015 | |
| PubMed ID | 26096057 | |
| PubMed Central ID | - | |
| DOI | 10.1038/ncomms8395 | |
| URL | - | |
| Relation | ||
| Gene | IAA11 IAA13 LRT2 TIR1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
