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| 文献 | ||
|---|---|---|
| 著者 | Raineri J., Wang S., Peleg Z., Blumwald E., Chan R.L. | |
| タイトル | The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress. | |
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Abstract: OsWRKY47 is a divergent rice transcription factor belonging to the group II of the WRKY family. A transcriptomic analysis of the drought response of transgenic rice plants expressing P SARK ::IPT, validated by qPCR, indicated that OsWRKY47 expression was induced under drought stress in P SARK ::IPT plants. A PCR-assisted site selection assay (SELEX) of recombinant OsWRKY47 protein showed that the preferred sequence bound in vitro is (G/T)TTGACT. Bioinformatics analyses identified a number of gene targets of OsWRKY47; among these two genes encode a Calmodulin binding protein and a Cys-rich secretory protein. Using Oswrk47 knockout mutants and transgenic rice overexpressing OsWRKY47 we show that the transcription of these putative targets were regulated by OsWRKY47. Phenotypic analysis carried out with transgenic rice plants showed that OsWRKY47 mutants displayed higher sensitivity to drought and reduced yield, while plants overexpressing OsWRKY47 were more tolerant. |
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| 掲載誌 | Plant Mol. Biol. | |
| Country | Argentine Republic | |
| 刊数 | 88(4-5) | |
| ページ | 401-13 | |
| 発刊年 | 2015 | |
| PubMed ID | 25957211 | |
| PubMed Central ID | - | |
| DOI | 10.1007/s11103-015-0329-7 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | WRKY47 _ _ | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
|
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
