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Author Zhang D., Liu M., Tang M., Dong B., Wu D., Zhang Z., Zhou B.
Title Repression of microRNA biogenesis by silencing of OsDCL1 activates the basal resistance to Magnaporthe oryzae in rice.
Abstract:
The RNaseIII enzyme Dicer-like 1 (DCL1) processes the microRNA biogenesis and plays a determinant role in plant development. In this study, we reported the function of OsDCL1 in the immunity to rice blast, the devastating disease caused by the fungal pathogen, Magnaporthe oryzae. Expression profiling demonstrated that different OsDCLs responded dynamically and OsDCL1 reduced its expression upon the challenge of rice blast pathogen. In contrast, miR162a predicted to target OsDCL1 increased its expression, implying a negative feedback loop between OsDCL1 and miR162a in rice. In addition to developmental defects, the OsDCL1-silencing mutants showed enhanced resistance to virulent rice blast strains in a non-race specific manner. Accumulation of hydrogen peroxide and cell death were observed in the contact cells with infectious hyphae, revealing that silencing of OsDCL1 activated cellular defense responses. In OsDCL1 RNAi lines, 12 differentially expressed miRNAs were identified, of which 5 and 7 were down- and up-regulated, respectively, indicating that miRNAs responded dynamically in the interaction between rice and rice blast. Moreover, silencing of OsDCL1 activated the constitutive expression of defense related genes. Taken together, our results indicate that rice is capable of activating basal resistance against rice blast by perturbing OsDCL1-dependent miRNA biogenesis pathway.
Journal Plant Sci.
Country China
Volume 237
Pages 24-32
Year 2015
PubMed ID 26089149
PubMed Central ID -
DOI 10.1016/j.plantsci.2015.05.002
URL -
Relation
Gene DCL1 DCL2A DCL2B DCL3A MIR156A MIR156B MIR156C MIR156D MIR156E MIR156F MIR156G MIR156I MIR156J MIR162A MIR166A MIR166B MIR166C MIR166D MIR166F MIR166N MIR167D MIR167E MIR167F MIR167G MIR167H MIR167I MIR167J MIR168A MIR169I MIR1862D MIR1882E MIR396E MIR5794 SDT SHO1
INSD -
Strain Wild Core Collection -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase