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| 著者 | Isshiki M., Matsuda Y., Takasaki A., Hann Ling Wong, Satoh H., Shimamoto K. | |
| タイトル | du3, a mRNA cap-binding protein gene, regulates amylose content in Japonica rice seeds | |
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Abstract: Five non-allelic dull (du) mutants have been isolated and characterized as low amylose mutants in rice (Oryza sativa) endosperm. Synthesis of amylose is controlled by Waxy the gene that encodes granule-bound starch synthase. We previously showed that the du1 and du2 proteins may encode general splicing regulators which affect the expression level and splicing efficiency of Waxyb (Wxb), an allele that has a single-base mutation in the 5′ splice site of intron 1. Here we report that the du3 mutant has also reduced the splicing efficiency of Wxb pre-mRNA. Furthermore, map-based cloning revealed that du3 encodes the rice homolog of a cap-binding protein 20 kD subunit (CBP20), a component of the heterodimeric nuclear cap-binding complex (CBC) playing a role in pre-mRNA splicing, RNA nuclear export, and nonsense-mediated decay. Transient expression of green fluorescent protein (GFP)-tagged du3 proteins in rice protoplasts resulted in localization to the nucleus showing a speckled pattern similar to the pattern displayed by other splicing factors. |
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| 掲載誌 | Plant Biotechnology | |
| Country | Japan | |
| 刊数 | 25(5) | |
| ページ | 483-487 | |
| 発刊年 | 2008 | |
| PubMed ID | - | |
| PubMed Central ID | - | |
| DOI | - | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | DU3 WX1 | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
