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Reference Detail
| Reference | ||
|---|---|---|
| Author | Byeon Y., Back K. | |
| Title | Molecular cloning of melatonin 2-hydroxylase responsible for 2-hydroxymelatonin production in rice (Oryza sativa). | |
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Abstract: Although melatonin biosynthetic genes from plants have been cloned, the melatonin catabolism mechanisms remain unclear. To clone the genes responsible for melatonin metabolism, we ectopically expressed 35 full-length cDNAs of rice 2-oxoglutarate-dependent dioxygenase (2-ODD) in Escherichia coli and purified the corresponding recombinant proteins. In vitro 2-ODD assays showed four independent 2-ODD proteins that were able to catalyze melatonin into 2-hydroxymelatonin, exhibiting melatonin 2-hydroxylase (M2H). These M2H proteins had peak activities at pH 8.0 and 30°C. The Km ranged from 121 mum to 371 mum with the Vmax ranging from 1.7 to 18.5 pkat/mg protein, respectively. The M2H enzyme activities were dependent on cofactors such as alpha-ketoglutarate, ascorbate, and Fe(2+), similar to the 2-ODD enzymes. M2H activity was inhibited by prohexadione-Ca, an inhibitor of 2-ODD, in a dose-dependent manner. M2H activity was high in the roots of rice seedlings, concurrent with high transcription levels of 2-ODD 21, suggesting that 2-ODD 21 was a major gene for M2H activity. Analogous to the high M2H activity in the roots, 2-hydroxymelatonin was found in large quantities in roots treated with melatonin. These results suggest that melatonin was metabolized into 2-hydroxymelatonin by the M2H genes in plants, but the physiological significance of 2-hydroxymelatonin remains to be examined in the future. |
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| Journal | J. Pineal Res. | |
| Country | South Korea | |
| Volume | 58(3) | |
| Pages | 343-51 | |
| Year | 2015 | |
| PubMed ID | 25728912 | |
| PubMed Central ID | - | |
| DOI | 10.1111/jpi.12220 | |
| URL | - | |
| Relation | ||
| Gene | 2ODD10 2ODD11 2ODD13 2ODD15 2ODD16 2ODD17 2ODD18 2ODD19 2ODD2 2ODD20 2ODD22 2ODD23 2ODD25 2ODD26 2ODD27 2ODD28 2ODD29 2ODD3 2ODD30 2ODD33 2ODD34 2ODD5 2ODD6 2ODD7 2ODD8 2ODD9 ACO5 EPE1 GA2OX11 GA2OX5 HIS1 ODD21 RL14 S3H SLC1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
