Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Zhang F., Sun Y., Pei W., Jain A., Sun R., Cao Y., Wu X., Jiang T., Zhang L., Fan X., Chen A., Sun S., Xu G. | |
| Title | Involvement of OsPht1;4 in phosphate acquisition, and mobilization facilitates embryo development in rice. | |
|
Abstract: Phosphate (Pi) transporters mediate acquisition, and transportation of Pi within plants. Here, we investigated the functions of OsPht1;4 (OsPT4), one of the thirteen members of the Pht1 family in rice. qRT-PCR analysis revealed strong expression of OsPT4 in roots and embryos, and OsPT4 promoter analysis using reporter genes confirmed these findings. Analysis using rice protoplasts showed that OsPT4 was localized to plasma membrane. OsPT4 complemented yeast mutant defective in Pi uptake, and also facilitated elevated accumulation of Pi in Xenopus oocytes. Further, OsPT4-genetically modified (GM) rice lines were generated by knockout/down, and overexpression of OsPT4. Pi concentrations in roots, and shoots were significantly lower, and higher in knockout/down, and overexpressing plants, respectively, compared to wild-type under different Pi regimes. (33) Pi uptake translocation assays corroborated altered acquisition, and mobilization of Pi in OsPT4-GM plants. The effects of altered expression levels of OsPT4 in GM plants were also evident on concentration of Pi, and size of embryo, and several attributes related to seed development. Overall, our results suggest that OsPT4 encodes a plasma membrane-localized Pi transporter facilitating acquisition, and mobilization of Pi, and also plays an important role in development of embryo in rice. This article is protected by copyright. All rights reserved. |
||
| Journal | Plant J. | |
| Country | China | |
| Volume | ||
| Pages | ||
| Year | 2015 | |
| PubMed ID | 25702710 | |
| PubMed Central ID | - | |
| DOI | 10.1111/tpj.12804 | |
| URL | - | |
| Relation | ||
| Gene | INO1 PT1 PT4 PT8 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
