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Author Ling S., Chen C., Wang Y., Sun X., Lu Z., Ouyang Y., Yao J.
Title The mature anther-preferentially expressed genes are associated with pollen fertility, pollen germination and anther dehiscence in rice.
Abstract:
The anthers and pollen grains are critical for male fertility and hybrid rice breeding. The development of rice mature anther and pollen consists of multiple continuous stages. However, molecular mechanisms regulating mature anther development were poorly understood. In this study, we have identified 291 mature anther-preferentially expressed genes (OsSTA) in rice based on Affymetrix microarray data. Gene Ontology (GO) analysis indicated that OsSTA genes mainly participated in metabolic and cellular processes that are likely important for rice anther and pollen development. The expression patterns of OsSTA genes were validated using real-time PCR and mRNA in situ hybridizations. Cis-element identification showed that most of the OsSTA genes had the cis-elements responsive to phytohormone regulation. Co-expression analysis of OsSTA genes showed that genes annotated with pectinesterase and calcium ion binding activities were rich in the network, suggesting that OsSTA genes could be involved in pollen germination and anther dehiscence. Furthermore, OsSTA RNAi transgenic lines showed male-sterility and pollen germination defects. The results suggested that OsSTA genes function in rice male fertility, pollen germination and anther dehiscence and established molecular regulating networks that lay the foundation for further functional studies.
Journal BMC Genomics
Country China
Volume 16
Pages 101
Year 2015
PubMed ID 25765586
PubMed Central ID 4340671
DOI 10.1186/s12864-015-1305-y
URL -
Relation
Gene 2R_MYB61 ABCG16 ABCG28 AGC26 AGPL4 ALDH2A AMY4A ANTH11 ANTH2 APT1 ARID2 B3DNA BAMY1 BBX15 BMY7 BZIP16 BZIP2 C2DP14 C2DP46 C2DP65 CBSDUF3 CHX1 CHX3 CHX4 CIPK05 CML3 CNGC16 CNGC17 CNGC6 DGK6 DHD4 DHHC26 ESD1 FBOX6 GATL4 GAUT7 GSL5 HAK6 HDA701 HSP16.9B IQM2 IQM6 JRL21 LFL1 LPR5 MADS27 MC3 MDR1 NAC10 NAC136 NHX6 NUDX12 ODD21 OSCA1.3 PDC3 PERK7 PGLP3 PLDbeta2 PLDdelta1 PME32 PMEI21 PPPCB PRD1 PT20 PUB13 RFPHC14 RKD10 RLCK189 RLCK342 ROPGEF2 SIP1;2 THI37 TIFY5 TIFY8 WSL4 XIO1 XTH8 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase