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Reference Detail
| Reference | ||
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| Author | Chen J., Wang Y., Wang F., Yang J., Gao M., Li C., Liu Y., Liu Y., Yamaji N., Ma J.F., Paz-Ares J., Nussaume L., Zhang S., Yi K., Wu Z., Wu P. | |
| Title | The rice CK2 kinase regulates trafficking of phosphate transporters in response to phosphate levels. | |
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Abstract: Phosphate transporters (PTs) mediate phosphorus uptake and are regulated at the transcriptional and posttranslational levels. In one key mechanism of posttranslational regulation, phosphorylation of PTs affects their trafficking from the endoplasmic reticulum (ER) to the plasma membrane. However, the kinase(s) mediating PT phosphorylation and the mechanism leading to ER retention of phosphorylated PTs remain unclear. In this study, we identified a rice (Oryza sativa) kinase subunit, CK2beta3, which interacts with PT2 and PT8 in a yeast two-hybrid screen. Also, the CK2alpha3/beta3 holoenzyme phosphorylates PT8 under phosphate-sufficient conditions. This phosphorylation inhibited the interaction of PT8 with PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1, a key cofactor regulating the exit of PTs from the ER to the plasma membrane. Additionally, phosphorus starvation promoted CK2beta3 degradation, relieving the negative regulation of PT phosphorus-insufficient conditions. In accordance, transgenic expression of a nonphosphorylatable version of OsPT8 resulted in elevated levels of that protein at the plasma membrane and enhanced phosphorus accumulation and plant growth under various phosphorus regimes. Taken together, these results indicate that CK2alpha3/beta3 negatively regulates PTs and phosphorus status regulates CK2alpha3/beta3. |
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| Journal | Plant Cell | |
| Country | China | |
| Volume | 27(3) | |
| Pages | 711-23 | |
| Year | 2015 | |
| PubMed ID | 25724641 | |
| PubMed Central ID | - | |
| DOI | 10.1105/tpc.114.135335 | |
| URL | - | |
| Relation | ||
| Gene | CK2ALPHA3 CK2BETA3 PHF1 PT2 PT8 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
