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Reference Detail
| Reference | ||
|---|---|---|
| Author | Chen Z., Gao X., Zhang J. | |
| Title | Alteration of osa-miR156e expression affects rice plant architecture and strigolactones (SLs) pathway. | |
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Abstract: Overexpressing osa - miR156e in rice produced a bushy mutant and osa - miR156e regulation of tillering may do this through the strigolactones (SLs) pathway. Appropriate downregulation of osa - miR156 expression contributed to the improvement of plant architecture. Tillering is one of the main determinants for rice architecture and yield. In this study, a bushy mutant of rice was identified with increased tiller number, reduced plant height, prolonged heading date, low seed setting, and small panicle size due to a T-DNA insertion which essentially elevated the expression of osa-miR156e. Transgenic plants with constitutive expression of osa-miR156e also had the bushy phenotype, which showed osa-miR156 may control apical dominance and tiller outgrowth via regulating the strigolactones signaling pathway. Furthermore, the extent of impaired morphology was correlated with the expression level of osa-miR156e. In an attempt to genetically improve rice architecture, ectopic expression of osa-miR156e under the GAL4-UAS system or OsTB1 promoter was conducted. According to agronomic trait analysis, pTB1:osa-miR156e transgenic plants significantly improved the grain yield per plant compared to plants overexpressing osa-miR156e, even though the yield was still inferior to the wild type, making it a very interesting albeit negative result. Our results suggested that osa-miR156 could serve as a potential tool for modifying rice plant architecture through genetic manipulation of the osa-miR156 expression level. |
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| Journal | Plant Cell Rep. | |
| Country | China | |
| Volume | 34(5) | |
| Pages | 767-81 | |
| Year | 2015 | |
| PubMed ID | 25604991 | |
| PubMed Central ID | - | |
| DOI | 10.1007/s00299-015-1740-x | |
| URL | - | |
| Relation | ||
| Gene | D14 D27 D3 MIR156E | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
