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| 文献 | ||
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| 著者 | Kwon C.T., Koo B.H., Kim D., Yoo S.C., Paek N.C. | |
| タイトル | Casein Kinases I and 2alpha Phosphorylate Oryza Sativa Pseudo-Response Regulator 37 (OsPRR37) in Photoperiodic Flowering in Rice. | |
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Abstract: flowering time (or heading date) is controlled by intrinsic genetic programs in response to environmental cues, such as photoperiod and temperature. Rice, a facultative shortday (SD) plant, flowers early in SD and late in long-day (LD) conditions. Casein kinases (CKs) generally act as positive regulators in many signaling pathways in plants. In rice, heading date 6 (Hd6) and Hd16 encode CK2alpha and CKI, respectively, and mainly function to delay flowering time. Additionally, the major LD-dependent floral repressors Hd2/Oryza sativa Pseudo-Response Regulator 37 (OsPRR37; hereafter PRR37) andGhd7 also confer strong photoperiod sensitivity. In floral induction, Hd16 acts upstream of Ghd7 and CKI interacts with and phosphorylates Ghd7. In addition, Hd6 and Hd16 also act upstream of Hd2. However, whether CKI and CK2alpha directly regulate the function of PRR37 remains unclear. Here, we use in vitro pull-down and in vivo bimolecular fluorescence complementation assays to show that CKI and CK2alpha interact with PRR37. We further use in vitro kinase assays to show that CKI and CK2alpha phosphorylate different regions of PRR37. Our results indicate that direct posttranslational modification of PRR37 mediates the genetic interactions between these two protein kinases and PRR37. The significance of CK-mediated phosphorylation for PRR37 and Ghd7 function is discussed. |
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| 掲載誌 | Mol. Cells | |
| Country | South Korea | |
| 刊数 | ||
| ページ | ||
| 発刊年 | 2014 | |
| PubMed ID | 25431424 | |
| PubMed Central ID | - | |
| DOI | 10.14348/molcells.2015.2254 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | GHD7 HD16 HD2 HD6 | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
