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Author Zhang X.a, Tang W.a, Liu J.b, Liu Y.a b
Title Co-expression of rice OsP5CS1 and OsP5CS2 genes in transgenic tobacco resulted in elevated proline biosynthesis and enhanced abiotic stress tolerance
In order to illustrate the specific gene functions of P5CS against abiotic stresses in rice, we constructed the overexpression binary pHB vector constitutively expressing two rice P5CS orthologs (OsP5CS1 and OsP5CS2), and then transformed it into tobacco using Agrobacterium-mediated transformation. Expression of the two transgenes (OsP5CS1 and OsP5CS2) was detected in the transgenic tobacco plants and a total of nine positive transgenic plants were obtained. The T2 generation of transgenic plants and wild tobacco seedlings were used for further abiotic stress experiments. Compared with the wild type plants, tobacco plants treated with three treatments (200 mmol/L NaCl, 300 mmol/L Mannitol and 300 mumol/L CuSO4) had average root length 1.3-2.3 times that of wild type plants, average fresh weight 1.9-3.9 times, average proline contents 1.8-3.2 times, average H2O2 contents 51% - 61%, and average MDA contents of transgenic plants 62% - 76% that of wild type plants. The results demonstrated that co-expression of OsP5CS1 and OsP5CS2 in transgenic tobacco increased proline biosynthesis and reduced oxidative damage of cells under various abiotic stress conditions. In addition, these results demonstrated the biological function of rice P5CS orthologs and their potential of bioengineering in plant tolerance to abiotic stresses.
Journal Chinese Journal of Applied and Environmental Biology
Country China
Volume 20
Pages 717-722
Year 2014
PubMed ID -
PubMed Central ID -
DOI 10.3724/SP.J.1145.2014.03010
Gene P5CS _
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
Stages in Each Organ
- Muant Lines (Gene)
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -