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Author Zhao S.P.a b, Ye X.Z.a, Shi W.M.c
Title Expression of OsAMT1 (1.1-1.3) in rice varieties differing in nitrogen accumulation
Abstract:
OsAMT is a high-affinity ammonium transporter responsible for NH4 + uptake by rice plants. To investigate the expression patterns of OsAMT in different genotypes in relation to nitrogen accumulation, we measured the expression of OsAMT1.1, OsAMT1.2, and OsAMT1.3 using Real-Time PCR (RT-PCR) in GD (higher N accumulation) and NG (lower N accumulation) seedlings of the Oryza sativa L. cultivar treated with 0.1 mM NH4NO3 and 2 mM NH4NO3. We found that the expression level of OsAMT1.1 was significantly higher than those of OsAMT1.2 and OsAMT1.3 in the roots treated with 0.1 mM NH4NO3, suggesting that OsAMT1.1 contributed the most to N accumulation among the three genes. In GD root, OsAMT1.1 had significantly higher expression levels when it was up-regulated by 0.1 mM NH4NO3 than when down-regulated by 2 mM NH4NO3. OsAMT1.1 was mainly found in GD roots treated with 0.1 mM NH4NO3. We conclude that the OsAMT1.1 in GD roots, which was significantly up-regulated by low N and down-regulated by high N, was the dominating factor in determining the higher N acquisition in GD than in NG at 0.1 mM NH4NO3. c 2014 Pleiades Publishing, Ltd.
Journal Russian Journal of Plant Physiology
Country China
Volume 61
Pages 707-713
Year 2014
PubMed ID -
PubMed Central ID -
DOI 10.1134/S1021443714040220
URL -
Relation
Gene AMT1;1 AMT1;2 AMT1;3
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
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