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著者 | Ke Y.G.a b, Yang Z.J.a, Yu S.W.b, Li T.F.b, Wu J.H.b, Gao H.b, Fu Y.P.c, Luo L.J.a b | |
タイトル | Characterization of OsDREB6 responsive to osmotic and cold stresses in rice | |
Abstract: We identified a new dehydration-responsive element-binding protein gene, OsDREB6, which is an A-6 subgroup member of the DREB subfamily, of AP2/EREBP proteins, in Oryza sativa ssp. Japonica cv. Nipponbare. Expression pattern analysis indicated that OsDREB6 was induced in response to dehydration, high salinity, and most importantly by cold stress (4°C). Analysis using the yeast one-hybrid system showed that OsDREB6 had transcriptional activation activity and bound specifically to the DRE ciselement. Overexpression of OsDREB6 increased tolerance to osmotic, high salt, and cold stresses in transgenic rice whereas, OsDREB6 double-stranded RNA interference (RNAi) lines were found to be more sensitive to these stresses than wild-type rice. Additionally, sugar, proline and catalase levels in OsDREB6 RNAi lines were significantly lower than in the corresponding OsDREB6 overexpression lines and control lines after PEG treatment. In contrast, malondialdehyde (MDA) levels were significantly higher in RNAi lines than in control and overexpression lines. Furthermore, expression levels of some stress-related genes were altered in the transgenic plants. The data suggests that OsDREB6 may participate in the stress response by mediating the expression of specific genes, and may also be involved plant metabolism. Together, these results indicated that OsDREB6 plays an important role in the response of plants to a wide range of osmotic stresses. c 2014 Korean Society of Plant Biologists and Springer-Verlag Berlin Heidelberg. |
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掲載誌 | Journal of Plant Biology | |
Country | China | |
刊数 | 57 | |
ページ | 150-161 | |
発刊年 | 2014 | |
PubMed ID | - | |
PubMed Central ID | - | |
DOI | 10.1007/s12374-013-0480-0 | |
URL | - | |
関連情報 | ||
遺伝子 | 2R_MYB36 BZIP23 DREB1B ERF50 NHX1 OSBZ8 P5CS PP2C53 PP2C6 SOS1 | |
INSD | - | |
系統 |
野生イネ コアコレクション |
- |
突然変異系統(遺伝研 Collection) | - | |
種子不稔系統 | - | |
致死変異体系統 | - | |
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
栽培イネ品種(遺伝研 Collection) | - | |
組織別発生段階 | - |