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Author Wu J.a d, Lee D.Y.b, Wang Y.a d, Kim S.T.c, Baek S.B.e, Kim S.G.e, Kang K.Y.a b
Title Protein profiles secreted from phylloplane of rice leaves free from cytosolic proteins: Application to study rice-Magnaporthe Oryzae interactions
Abstract:
Magnaporthe oryzae causes rice blast disease, which threatens global rice (. Oryzae sativa) production. We identified proteins secreted during rice blast infection by performing proteomics analysis of rice phylloplane secreted proteins. Apoplastic proteins were analyzed by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. The biological functions of proteins induced by M.oryzae infection were related to metabolism (47%), stress/defense (36%), and proteolysis (13%). Most secreted proteins were predicted to retain a signal peptide and were highly expressed in incompatible interactions. A domain of unknown function 26 (OsDUF26) apoplastic protein was most abundantly secreted in response to rice blast infection. Transgenic rice and Arabidopsis plants expressing OsDUF26-promoter::GFP were generated to assess the role of OsDUF26 in response to biotic stress. The OsDUF26 promoter was activated in response to Xanthomonas oryzae, M. oryzae, and fungal elicitor. This work shows that plant apoplastic proteins might play key roles in plant responses to biotic stress.
Journal Physiol. Mol. Plant Pathol.
Country South Korea
Volume 88
Pages 28-35
Year 2014
PubMed ID -
PubMed Central ID -
DOI 10.1016/j.pmpp.2014.08.003
URL -
Relation
Gene ALPHACA3 BG3 CHT1 GH5BG GLP4-1 GNS1 GNS10 PME7 POX22.3 POXN PRX117 RMC SUB30 SUB64 _ _ _ _ _ _ _ _ _ _ _ _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase