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Reference Detail
| Reference | ||
|---|---|---|
| Author | Lee G.W., Lee S., Chung M.S., Jeong Y.S., Chung B.Y. | |
| Title | Rice terpene synthase 20 (OsTPS20) plays an important role in producing terpene volatiles in response to abiotic stresses. | |
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Abstract: This study examined the volatile terpenes produced by rice seedlings in response to oxidative stress induced by various abiotic factors. Solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) analyses revealed that when exposed to UV-B radiation, rice seedlings emitted a bouquet of monoterpene mixtures in a time-dependent manner. The mixtures comprised limonene, sabinene, myrcene, alpha-terpinene, beta-ocimene, gamma-terpinene, and alpha-terpinolene. Among them, (S)-limonene was the most abundant volatile, discriminated by chiral SPME-GC-MS. The volatile profiles collected from rice plants treated with both gamma-irradiation and H2O2 were identical to those observed in the UV-B irradiated plants, thus indicating that the volatile mixtures were specifically produced in response to oxidative stress, particularly in the presence of H2O2. Using a reverse genetics approach, we isolated full-length Rice terpene synthase 20 (OsTPS20, 599 amino acids, 69.39 kDa), which was further characterized as an (S)-limonene synthase by removing the N-terminal signal peptide (63 amino acids) of the protein. The recombinant OsTPS20 protein catalyzed the conversion of geranyl diphosphate to (S)-limonene and other minor monoterpenes, essentially covering all of the volatile compounds detected from the plant. Moreover, qRT-PCR revealed that the transcript levels of OsTPS20 were significantly induced in response to oxidative stress, thereby suggesting that OsTPS20 plays a major role in producing terpene volatiles during abiotic stress. Detailed biochemical analyses and the unusual domain characteristics of OsTPS20 are also discussed in this report. |
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| Journal | Protoplasma | |
| Country | South Korea | |
| Volume | ||
| Pages | ||
| Year | 2014 | |
| PubMed ID | 25430981 | |
| PubMed Central ID | - | |
| DOI | 10.1007/s00709-014-0735-8 | |
| URL | - | |
| Relation | ||
| Gene | CPS1 CPS2 CPS4 KS1 KS2 KS3 KS4 KS5 KS6 KS7 KS8 KSL10 TPS12 TPS13 TPS14 TPS17 TPS18 TPS19 TPS2 TPS20 TPS21 TPS22 TPS23 TPS24 TPS29 TPS3 TPS30 TPS44 _ _ _ _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
