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| 著者 | Nayar S., Kapoor M., Kapoor S. | |
| タイトル | Post-translational regulation of rice MADS29 function: homodimerization or binary interactions with other seed-expressed MADS proteins modulate its translocation into the nucleus. | |
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Abstract: OsMADS29 is a seed-specific MADS-box transcription factor that affects embryo development and grain filling by maintaining hormone homeostasis and degradation of cells in the nucellus and nucellar projection. Although it has a bipartite nuclear localization signal (NLS) sequence, the transiently expressed OsMADS29 monomer does not localize specifically in the nucleus. Dimerization of the monomers alters the intracellular localization fate of the resulting OsMADS29 homodimer, which then translocates into the nucleus. By generating domain-specific deletions/mutations, we show that two conserved amino acids (lysine(23) and arginine(24)) in the NLS are important for nuclear localization of the OsMADS29 homodimer. Furthermore, the analyses involving interaction of OsMADS29 with 30 seed-expressed rice MADS proteins revealed 19 more MADS-box proteins, including five E-class proteins, which interacted with OsMADS29. Eleven of these complexes were observed to be localized in the nucleus. Deletion analysis revealed that the KC region (K-box and C-terminal domain) plays a pivotal role in homodimerization. These data suggest that the biological function of OsMADS29 may not only be regulated at the level of transcription and translation as reported earlier, but also at the post-translational level by way of the interaction between OsMADS29 monomers, and between OsMADS29 and other MADS-box proteins. |
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| 掲載誌 | J. Exp. Bot. | |
| Country | India | |
| 刊数 | 65(18) | |
| ページ | 5339-50 | |
| 発刊年 | 2014 | |
| PubMed ID | 25096923 | |
| PubMed Central ID | 4157715 | |
| DOI | 10.1093/jxb/eru296 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | COG1 FST LHS1 MADS18 MADS22 MADS24 MADS25 MADS26 MADS31 MADS33 MADS5 MADS51 MADS55 MADS56 MADS66 MADS7 MFO1 PAP2 RAG RAP1B | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
|
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
