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Author Wei S., Hu W., Deng X., Zhang Y., Liu X., Zhao X., Luo Q., Jin Z., Li Y., Zhou S., Sun T., Wang L., Yang G., He G.
Title A rice calcium-dependent protein kinase OsCPK9 positively regulates drought stress tolerance and spikelet fertility.
Abstract:
In plants, calcium-dependent protein kinases (CDPKs) are involved in tolerance to abiotic stresses and in plant seed development. However, the functions of only a few rice CDPKs have been clarified. At present, it is unclear whether CDPKs also play a role in regulating spikelet fertility. We cloned and characterized the rice CDPK gene, OsCPK9. OsCPK9 transcription was induced by abscisic acid (ABA), PEG6000, and NaCl treatments. The results of OsCPK9 overexpression (OsCPK9-OX) and OsCPK9 RNA interference (OsCPK9-RNAi) analyses revealed that OsCPK9 plays a positive role in drought stress tolerance and spikelet fertility. Physiological analyses revealed that OsCPK9 improves drought stress tolerance by enhancing stomatal closure and by improving the osmotic adjustment ability of the plant. It also improves pollen viability, thereby increasing spikelet fertility. In OsCPK9-OX plants, shoot and root elongation showed enhanced sensitivity to ABA, compared with that of wild-type. Overexpression and RNA interference of OsCPK9 affected the transcript levels of ABA- and stress-responsive genes. Our results demonstrated that OsCPK9 is a positive regulator of abiotic stress tolerance, spikelet fertility, and ABA sensitivity.
Journal BMC Plant Biol.
Country China
Volume 14
Pages 133
Year 2014
PubMed ID 24884869
PubMed Central ID 4036088
DOI 10.1186/1471-2229-14-133
URL -
Relation
Gene BZIP23 BZIP72 CDPK9 LEA3-1 NAC45 NAC6 P5CS RAB16B TRAB1 _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase