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Reference Detail
| Reference | ||
|---|---|---|
| Author | Liu H., Li X., Xiao J., Wang S. | |
| Title | A convenient method for simultaneous quantification of multiple phytohormones and metabolites: application in study of rice-bacterium interaction. | |
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Abstract: ABSTRACT: Simultaneous analysis of multiple functional-related phytohormones and their metabolites will improve our understanding of interactions among different hormones in the same biologic process. A method was developed for simultaneous quantification of multiple phytohormones, abscisic acid, indole-3-acetic acid (IAA), jasmonic acid (JA), and salicylic acid, hormone conjugates, IAA-aspartic acid, JA-isoleucine, and methyl JA, and phytoalexins, momilactone A, naringenin, and sakuranetin. This method combines a convenient procedure for preparing filtrated crude extracted samples and a sensitive quantification assay using ultra fast liquid chromatography-electrospray ionization tandem mass spectrometry (UFLC-ESI-MS). With this method, we determined the dynamic profiles of defense-related phytohormones, hormone metabolites, and phytoalexins in the interaction of rice with Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial blight, one of the most devastating diseases of rice worldwide. This UFLC-ESI-MS method is convenient, sensitive, reliable, and inexpensive for quantification of multiple phytohormones and metabolites compared to current methods. The results obtained by application of this method in studying rice-bacterial interaction provide a basis for understanding the molecular mechanisms of rice defense responses. |
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| Journal | Plant Methods | |
| Country | China | |
| Volume | 8(1) | |
| Pages | 2 | |
| Year | 2012 | |
| PubMed ID | 22243810 | |
| PubMed Central ID | 3274484 | |
| DOI | - | |
| URL | - | |
| Relation | ||
| Gene | ABA1 AO1 AOS2 CHS CPS4 ICS1 KS4 NCED1 NCED1 NIT1 PAL1 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
