更新情報研究支援ツール - 文献 - 詳細
文献詳細
| 文献 | ||
|---|---|---|
| 著者 | Wang C., Ye J., Tang W., Liu Z., Zhu C., Wang M., Wan J. | |
| タイトル | Loop Nucleotide Polymorphism in a Putative miRNA precursor Associated with seed length in Rice (Oryza sativa L.). | |
|
Abstract: The terminal loop region of primary miRNA transcripts is an important determinant controlling miRNA function in human, animals and plants. However, the effects of polymorphisms in loop region of miRNA precusors on phenotypic variation have not been reported, especially on agronomic traits in rice. From rice japonica variety Koshihikari and indica Guichao2, we isolated a precursor of putative miRNA osa-MIR2923a. We detected a GG/AA polymorphism in the loop structure from japonica and indica rice varieties, which was corresponding to japonica/indica rice differentiation. By using high-resolution melting (HRM) analysis, we measured the polymorphisms in a RIL (recombinant inbred lines) population derived from japonica variety Koshihikari and indica Guichao2. We found that the GG/AA polymorphism in the osa-MIR2923a loop was correlated to grain length and length-width ratio. We further found the significant association between seed length and GG/AA polymorphism in a population consisting of 72 rice landraces. Three targets were predicted, whose expressions showed significant differences between the two varieties. Our results suggested that the putative miRNA precursor and the three target genes could play functional roles for indica/japonica seed differentiation. |
||
| 掲載誌 | Int. J. Biol. Sci. | |
| Country | China | |
| 刊数 | 9(6) | |
| ページ | 578-86 | |
| 発刊年 | 2013 | |
| PubMed ID | 23847440 | |
| PubMed Central ID | 3708038 | |
| DOI | 10.7150/ijbs.6357 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | ALPHACA10 BHC MIR2923A RIM2-M53 | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
|
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
