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| 著者 | Chen H., Cheng Z., Ma X., Wu H., Liu Y., Zhou K., Chen Y., Ma W., Bi J., Zhang X., Guo X., Wang J., Lei C., Wu F., Lin Q., Liu Y., Liu L., Jiang L. | |
| タイトル | A knockdown mutation of YELLOW-GREEN LEAF2 blocks chlorophyll biosynthesis in rice. | |
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Abstract: An insert mutation of YELLOW - GREEN LEAF2 , encoding Heme oxygenase 1 , results in significant reduction of its transcript levels, and therefore impairs chlorophyll biosynthesis in rice. Heme oxygenase (HO) in higher plants catalyzes the degradation of heme to synthesize phytochrome precursor and its roles conferring the photoperiodic control of flowering in rice have been revealed. However, its involvement in regulating rice chlorophyll (Chl) synthesis is not fully explored. In this study, we isolated a rice mutant named yellow-green leaf 2 (ygl2) from a (60)Co-irradiated population. Normal grown ygl2 seedlings showed yellow-green leaves with reduced contents of Chl and tetrapyrrole intermediates whereas an increase of Chl a/b ratio. Ultrastructural analyses demonstrated grana were poorly stacked in ygl2 mutant, resulting in underdevelopment of chloroplasts. The ygl2 locus was mapped to chromosome 6 and isolated via map-based cloning. Sequence analysis indicated that it encodes the rice HO1 and its identity was verified by transgenic complementation test and RNA interference. A 7-Kb insertion was found in the first exon of ygl2/HO1, resulting in significant reduction of ygl2 expressions in the ygl2 mutant. ygl2 was constitutively expressed in a variety of rice tissues with the highest levels in leaves and regulated by temperature. In addition, we found expression levels of some genes associated with Chl biosynthesis and photosynthesis were concurrently altered in ygl2 mutant. These results provide direct evidence that ygl2 has a vital function in rice Chl biosynthesis. |
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| 掲載誌 | Plant Cell Rep. | |
| Country | China | |
| 刊数 | 32(12) | |
| ページ | 1855-67 | |
| 発刊年 | 2013 | |
| PubMed ID | 24043333 | |
| PubMed Central ID | - | |
| DOI | 10.1007/s00299-013-1498-y | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | CAB1R CAB2R CHLH ELL FGL PSAA PSBA SE5 YGL1 YGL10 YGL98 | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
