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Author Song Y.a, Cui J.a, Zhang H.b, Wang G.a, Zhao F.J.c, Shen Z.a
Title Proteomic analysis of copper stress responses in the roots of two rice (Oryza sativa L.) varieties differing in Cu tolerance
Abstract:
Background and aims: Copper (Cu) is an essential micronutrient required for growth and development of plants. However, excess Cu is toxic to plants. To understand the mechanisms involved in copper stress response, a proteomic approach was used to investigate the differences in Cu stress-induced protein expression between a Cu-tolerant variety (B1139) and a Cu-sensitive one (B1195) of rice. Methods: Rice seedlings were exposed to 8 muM Cu for 3 days, with plants grown in the normal nutrient solution containing 0. 32 muM Cu serving as the control. Proteins were extracted from the roots and separated by two-dimensional PAGE. Thirty four proteins were identified using MALDI-TOF mass spectrometry. Results: Thirty-four protein spots were found to be differently expressed in the Cu-stressed roots in at least one variety of rice, including those involved in antioxidative defense, redox regulation, stress response, sulfur and glutathione (GSH) metabolism, carbohydrate metabolism, signal transduction, and some other proteins with various functions. Nine proteins, including putative cysteine synthase, probable serine acetyltransferase 3, L-ascorbate peroxidase 1, putative glutathione S-transferase 2, and thioredoxin-like 3-3, exhibited a greater increase in response to Cu stress in the Cu-tolerant variety B1139 compared with the Cu-sensitive variety B1195. Conclusion: The majority of the proteins showing differential expression in response to Cu exposure are involved in the redox regulation, and sulfur and GSH metabolism, suggesting that these proteins, together with antioxidant enzymes, play an important role in the detoxification of excess Cu and maintaining cellular homeostasis.
Journal Plant Soil
Country China
Volume 366
Pages 647-658
Year 2013
PubMed ID -
PubMed Central ID -
DOI 10.1007/s11104-012-1458-2
URL -
Relation
Gene APX1 APX2 C3H21 GH3-10 GLYI-11 GRX28 GSTL3 GSTU2 HSP82B PDC2 RBBI3-3 RCS1 RF1 RMC RSOSPR10 TID1 TUBA1 TUBA3 _ _ _ _ _ _ _ _ _ _ _ _ _ _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase