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Author Zhang C.Y.a b, Wang N.N.c, Zhang Y.H.a, Feng Q.Z.a, Yang C.W.a, Liu B.a
Title DNA methylation involved in proline accumulation in response to osmotic stress in rice (Oryza sativa)
Proline accumulation is involved in plant osmotic stress tolerance. Given that DNA methylation is related to almost all metabolic processes through regulation of gene expression, we suspected that this epigenetic modification and proline biosynthesis are probably related. To test this, we investigated whether osmotic stress-induced proline accumulation is associated with DNA methylation modifications in rice. We assessed DNA methylation and expression of 3 key genes (P5CR, P5CS, and delta-OAT) involved in proline biosynthesis, and measured proline content under both osmotic stress (15% polyethylene glycol) and control conditions. After osmotic stress, selfed progenies of osmotic-stressed plants accumulated higher concentrations of proline in leaves under both normal conditions and under osmotic stress than the unstressed control plants. Concomitantly, under osmotic stress, the selfed progeny plants showed higher expression levels of P5CS and delta-OAT than the control. This up-regulated expression was stably inherited by the subsequent generation. Methylation-sensitive Southern blotting indicated that 2 of the 3 genes, P5CS and delta-OAT, had greater DNA demethylation in the selfed progenies than in the control. Apparently DNA demethylation facilitated proline accumulation by up-regulating expression of the P5CS and delta-OAT genes in response to osmotic stress.
Journal Genetics and Molecular Research
Country China
Volume 12
Pages 1269-1277
Year 2013
PubMed ID 23661451
PubMed Central ID -
DOI 10.4238/2013.april.17.5
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
Stages in Each Organ
- Muant Lines (Gene)
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -