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Author Fang Y., You J., Xie K., Xie W., Xiong L.
Title Systematic sequence analysis and identification of tissue-specific or stress-responsive genes of NAC transcription factor family in rice.
NAM, ATAF, and CUC (NAC) transcription factors comprise a large plant-specific gene family and a few members of this family have been characterized for their roles in plant growth, development, and stress tolerance. In this study, systematic sequence analysis revealed 140 putative NAC or NAC-like genes (ONAC) in rice. Phylogenetic analysis suggested that NAC family can be divided into five groups (I-V). Among them, all the published development-related genes fell into group I, and all the published stress-related NAC genes fell into the group III (namely stress-responsive NAC genes, SNAC). Distinct compositions of the putative motifs were revealed on the basis of NAC protein sequences in rice. Most members contained a complete NAC DNA-binding domain and a variable transcriptional regulation domain. Sequence analysis, together with the organization of putative motifs, indicated distinct structures and potential diverse functions of NAC family in rice. Yeast one-hybrid analysis confirmed that 12 NAC proteins representing different motif compositions can bind the NAC core DNA-binding site. Real-time polymerase chain reaction (PCR) analysis revealed 12 genes with different tissue-specific (such as callus, root, stamen, or immature endosperm) expression patterns, suggesting that these genes may play crucial regulatory roles during growth and development of rice. The expression levels of this family were also checked under various abiotic stresses including drought, salinity, and low temperature. A preliminary check based on our microarray data suggested that more than 40 genes of this family were responsive to drought and/or salt stresses. Among them, 20 genes were further investigated for their stress responsiveness in detail by real-time PCR analysis. Most of these stress-responsive genes belonged to the group III (SNAC). Considering the fact that a very limited number of genes of the NAC family have been characterized, our data provide a very useful reference for functional analysis of this family in rice.
Journal Mol. Genet. Genomics
Country China
Volume 280(6)
Pages 547-63
Year 2008
PubMed ID 18813954
PubMed Central ID -
DOI 10.1007/s00438-008-0386-6
Gene BET1 CUC1 CUC3 NAC1 NAC10 NAC100 NAC101 NAC102 NAC103 NAC104 NAC105 NAC106 NAC108 NAC110 NAC111 NAC112 NAC113 NAC114 NAC115 NAC116 NAC117 NAC118 NAC119 NAC12 NAC120 NAC121 NAC122 NAC123 NAC124 NAC125 NAC126 NAC127 NAC128 NAC129 NAC13 NAC130 NAC131 NAC133 NAC134 NAC135 NAC136 NAC137 NAC138 NAC139 NAC14 NAC140 NAC15 NAC16 NAC17 NAC18 NAC19 NAC20 NAC21 NAC22 NAC24 NAC25 NAC26 NAC28 NAC29 NAC3 NAC30 NAC31 NAC32 NAC33 NAC34 NAC35 NAC36 NAC37 NAC38 NAC4 NAC40 NAC41 NAC42 NAC43 NAC44 NAC45 NAC46 NAC47 NAC49 NAC5 NAC50 NAC51 NAC52 NAC53 NAC55 NAC56 NAC57 NAC58 NAC59 NAC6 NAC6 NAC60 NAC61 NAC62 NAC63 NAC64 NAC65 NAC66 NAC69 NAC7 NAC70 NAC71 NAC72 NAC73 NAC75 NAC76 NAC77 NAC78 NAC79 NAC8 NAC80 NAC82 NAC83 NAC84 NAC85 NAC86 NAC87 NAC88 NAC89 NAC90 NAC91 NAC94 NAC95 NAC96 NAC97 NAC98 NAC99 NAM ONAC2 ONAC300 RIM1 TIL1 TMS5 Y37 YL3 _ _ _ _ _
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
Stages in Each Organ
- Muant Lines (Gene)
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -