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Reference Detail
| Reference | ||
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| Author | Mustafiz A., Ghosh A., Tripathi A.K., Kaur C., Ganguly A.K., Bhavesh N.S., Tripathi J.K., Pareek A., Sopory S.K., Singla-Pareek SL. | |
| Title | A unique Ni(2+) -dependent and methylglyoxal-inducible rice glyoxalase I possesses a single active site and functions in abiotic stress response. | |
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Abstract: Glyoxalase system constitutes the major pathway for detoxification of metabolically produced cytotoxin methylglyoxal (MG), into a non-toxic metabolite D-Lactate. glyoxalase I (GLY I) is an evolutionarily conserved metalloenzyme requiring divalent metal ions for its activity, Zn(2+) in case of eukaryotes, or Ni(2+) for enzymes of prokaryotic origin. Plant glyoxalase I proteins are part of a multi-member family, however not much is known about their physiological function, structure, and metal dependency. In this study, we report a unique glyoxalase I (OsGLYI-11.2) from rice which requires Ni(2+) for its activity. Its biochemical, structural and functional characterization revealed it to be a monomeric enzyme, possessing a single Ni(2+) coordination site despite containing two glyoxalase I domains. Requirement of Ni(2+) as a cofactor by an enzyme involved in cellular detoxification suggests an essential role for this otherwise toxic heavy metal in stress response. Intriguingly, the expression of OsGLYI-11.2 was found to be highly substrate-inducible suggesting an important mode of regulation of its cellular levels. Heterologous expression of OsGLYI-11.2 in E. coli and model plant tobacco resulted in improved adaptation to various abiotic stresses due to increased scavenging of MG, lower Na(+) /K(+) ratio and maintenance of reduced glutathione levels. Together, our results suggest interesting links between MG cellular levels, its detoxification by glyoxalase I, and Ni(2+) - the heavy metal cofactor of OsGLYI-11.2, vis-à-vis stress response and adaptation in plants. This article is protected by copyright. All rights reserved. |
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| Journal | Plant J. | |
| Country | India | |
| Volume | 78(6) | |
| Pages | 951-63 | |
| Year | 2014 | |
| PubMed ID | 24661284 | |
| PubMed Central ID | - | |
| DOI | 10.1111/tpj.12521 | |
| URL | - | |
| Relation | ||
| Gene | GLYI-11 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
