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| 著者 | Wang S., Xu Y., Li Z., Zhang S., Lim J.M., Lee K.O., Li C., Qian Q., Jiang D.A., Qi Y. | |
| タイトル | OsMOGS is required for N-glycan formation and auxin-mediated root development in rice (Oryza sativa L.). | |
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Abstract: N-glycosylation is a major modification of glycoproteins in eukaryotic cells. In Arabidopsis, great progress has been made in functional analysis of N-glycan production, however there are few studies in monocotyledons. Here, we characterized a rice (Oryza sativa L.) OsMOGS mutant with shortened roots and isolated a gene that coded a putative mannosyl-oligosaccharide glucosidase (OsMOGS), an ortholog of alpha-glucosidase I in Arabidopsis, which trims the terminal glucosyl residue of the oligosaccharide chain of nascent peptides in the endoplasmic reticulum (ER). OsMOGS is strongly expressed in rapidly cell-dividing tissues and OsMOGS protein is localized in the ER. Mutation of OsMOGS entirely blocked N-glycan maturation and inhibited high-mannose N-glycan formation. The OsMOGS mutant exhibited severe defects in root cell division and elongation, resulting in a short-root phenotype. In addition, OsMOGS plants had impaired root hair formation and elongation, and reduced root epidemic cell wall thickness due to decreased cellulose synthesis. Further analysis showed that auxin content and polar transport in OsMOGS roots were reduced due to incomplete N-glycosylation of the B subfamily of ATP-binding cassette transporter proteins (ABCBs). Our results demonstrate that involvement of OsMOGS in N-glycan formation is required for auxin-mediated root development in rice. |
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| 掲載誌 | Plant J. | |
| Country | China | |
| 刊数 | 78(4) | |
| ページ | 632-45 | |
| 発刊年 | 2014 | |
| PubMed ID | 24597623 | |
| PubMed Central ID | - | |
| DOI | 10.1111/tpj.12497 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | IAA1 MDR13 MDR6 SAUR39 _ | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
