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Author Zhou Y., Liu L., Huang W., Yuan M., Zhou F., Li X., Lin Y.
Title Overexpression of OsSWEET5 in Rice Causes Growth Retardation and Precocious Senescence.
Abstract:
As a novel sugar transporter family, SWEETs play important roles in plant growth and development. Here, we characterized a SWEET gene named OsSWEET5 through its overexpression in rice. Heterologous expression assay indicated that OsSWEET5 encoded a galactose transporter in yeast. OsSWEET5-overexpressing plants displayed the phenotypes of growth retardation and precocious senescence at seedling stage. GC-MS analysis showed that the sugar levels were largely altered in the leaves of the OsSWEET5-overexpressing plants. Molecular analysis revealed that these phenotypes might be due to the transcriptional changes of the genes involved in sugar metabolism and transport. In addition, the transgenic plants showed a lower level of auxin with altered transcription of genes involved in auxin signaling and translocation pathways. However, no obvious phenotype was observed between the amiRNA-OsSWEET5 transgenic lines and WT plants, which could be a result of the functional redundancy of the galactose transporters in rice. Taken together, our findings suggest that OsSWEET5 plays a crucial role in regulating the crosstalk between sugar and auxin in rice.
Journal PLoS ONE
Country China
Volume 9(4)
Pages e94210
Year 2014
PubMed ID 24709840
PubMed Central ID 3978035
DOI 10.1371/journal.pone.0094210
URL -
Relation
Gene GH3-2 IAA24 IAA31 PIN1B SAUR39 SUT1 SUT2 SWEET5 TMT1 TMT2
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain 		-
Stages in Each Organ
- Muant Lines (Gene) 		-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase