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Reference Detail
| Reference | ||
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| Author | Naeem M.a c, Freed S.b, Quan Z.G.a | |
| Title | Molecular genetic studies of heading date gene OsMADS50 by using single segment substitution lines in Oryza sativa | |
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Abstract: heading date is multifarious, polygenic character responsible for the adaptation of rice to various agro-climatic regions. W23-3-8-9-27-82 and W08-16-3-2 are Single Segment Substitution lines containing OsMADS50 on third chromosome of rice. Segregating (F2) populations of HJX74 × W23-3-8-9-27-82, HJX74 × W08-16-3-2 were utilized in the genetic study of OsMDAS50 gene. From the whole genomic sequence of OsMADS50 gene three functional markers PSM908, PSM909 and PSM910 were developed and utilized for genetic analysis. Significant Chi square values for each population and zero cM genetic distance between OsMADS50 gene and PSM908 proved the linkage with heading date trait. Three different phenotypes of OsMADS50 gene were identified varying in days to heading but linked with PSM908. Expression analysis of OsMADS50, Hd3a, RFT1 revealed strongest expression in W23-3-8-9-27-82, while week expression was found in W08-16-3-2 and weakest expression was observed in HJX74, resulting in earliest heading of W23-3-8-9-27-82, followed by W08-16-3-2 and HJX74. These three different genotypes of OsMADS50 are due to three different naturally occurring alleles of OsMADS50 found in corresponding parental varieties, Lemont's being headed earliest followed by IR64's and least one was HJX74's. |
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| Journal | Int J Agric Biol | |
| Country | China | |
| Volume | 15 | |
| Pages | 631-639 | |
| Year | 2013 | |
| PubMed ID | - | |
| PubMed Central ID | - | |
| DOI | - | |
| URL | - | |
| Relation | ||
| Gene | HD3A MADS50 RFT1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
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Stages in Each Organ - Muant Lines (Gene) |
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| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
