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| 文献 | ||
|---|---|---|
| 著者 | Koshiba T.a, Hirose N.a, Mukai M.a, Yamamura M.a, Hattori T.a d, Suzuki S.a, Sakamoto M.b, Umezawa T.a c | |
| タイトル | Characterization of 5-hydroxyconiferaldehyde O-methyltransferase in Oryza sativa | |
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Abstract: Little is known regarding syringyl lignin biosynthesis in rice (Oryza sativa L. cv. Nipponbare). In the present study, the role of rice cafeic acid O-methyltransferase (OsCOMT1, Q6ZD89), was examined. Te recombinant OsCOMT1 catalyzed the 5-Omethylation of 5-hydroxyferulate (5-HFA) and 5-hydroxyconiferaldehyde (5-HCAld). 5-HCAld inhibited 5-HFA methylation by this O-methyltransferase (OMT), while 5-HFA mitigated self-inhibition in 5-HCAld methylation. A rice plant in which OsCOMT1 expression was downregulated exhibited weakened cell wall staining with Wiesner reagent in vascular bundle cells and sclerenchyma tissue, compared with wild-type plants. Te lignin content of transgenic rice plants was decreased and the syringyl lignin content reduced largely compared with that of the wild type. Taken together, these data indicated that OsCOMT1 functioned as a 5-HCAld OMT (OsCAldOMT1) in the biosynthetic pathway to syringyl lignin. |
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| 掲載誌 | Plant Biotechnology | |
| Country | Japan | |
| 刊数 | 30 | |
| ページ | 157-167 | |
| 発刊年 | 2013 | |
| PubMed ID | - | |
| PubMed Central ID | - | |
| DOI | 10.5511/plantbiotechnology.13.0219a | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | 4CL3 4CL5 ACS9 C3H1 C4H1 C4H2 CAD8C CCR19 CCR20 CCR21 COMTL4 F5HL2 GH2 HCT1 HCT2 PAL PAL1 ROMT9 _ _ _ _ _ _ | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
