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Reference Detail
| Reference | ||
|---|---|---|
| Author | Koshiba T.a, Hirose N.a, Mukai M.a, Yamamura M.a, Hattori T.a d, Suzuki S.a, Sakamoto M.b, Umezawa T.a c | |
| Title | Characterization of 5-hydroxyconiferaldehyde O-methyltransferase in Oryza sativa | |
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Abstract: Little is known regarding syringyl lignin biosynthesis in rice (Oryza sativa L. cv. Nipponbare). In the present study, the role of rice cafeic acid O-methyltransferase (OsCOMT1, Q6ZD89), was examined. Te recombinant OsCOMT1 catalyzed the 5-Omethylation of 5-hydroxyferulate (5-HFA) and 5-hydroxyconiferaldehyde (5-HCAld). 5-HCAld inhibited 5-HFA methylation by this O-methyltransferase (OMT), while 5-HFA mitigated self-inhibition in 5-HCAld methylation. A rice plant in which OsCOMT1 expression was downregulated exhibited weakened cell wall staining with Wiesner reagent in vascular bundle cells and sclerenchyma tissue, compared with wild-type plants. Te lignin content of transgenic rice plants was decreased and the syringyl lignin content reduced largely compared with that of the wild type. Taken together, these data indicated that OsCOMT1 functioned as a 5-HCAld OMT (OsCAldOMT1) in the biosynthetic pathway to syringyl lignin. |
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| Journal | Plant Biotechnology | |
| Country | Japan | |
| Volume | 30 | |
| Pages | 157-167 | |
| Year | 2013 | |
| PubMed ID | - | |
| PubMed Central ID | - | |
| DOI | 10.5511/plantbiotechnology.13.0219a | |
| URL | - | |
| Relation | ||
| Gene | 4CL3 4CL5 ACS9 C3H1 C4H1 C4H2 CAD8C CADL CCR19 CCR20 CCR21 COMTL4 F5HL2 GH2 HCT1 HCT2 HCT3 PAL PAL1 ROMT9 _ _ _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
