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Reference Detail
| Reference | ||
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| Author | Park H.L., Lee S.W., Jung K.H., Hahn T.R., Cho M.H. | |
| Title | Transcriptomic analysis of UV-treated rice leaves reveals UV-induced phytoalexin biosynthetic pathways and their regulatory networks in rice. | |
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Abstract: Rice produces diterpenoid and flavonoid phytoalexins for defense against pathogen attack. The production of phytoalexins in rice is also induced by UV-irradiation. To understand the metabolic networks involved in UV-induced phytoalexin biosynthesis and their regulation, phytochemical and transcriptomic analyses of UV-treated rice leaves were performed. In response to UV treatment, the accumulation of flavonoids was observed in rice leaves, which may serve as antioxidants against UV-induced oxidative stress. The phytochemical analysis confirmed sakuranetin accumulation and also demonstrated the induction of phenylamide synthesis in rice leaves by UV-irradiation. Transcriptomic analysis established that aromatic amino acid biosynthetic genes were immediately up-regulated after UV treatment. The genes involved in the phenylpropanoid pathway and flavonoid biosynthesis were also up-regulated. These findings suggest that the aromatic amino acid and flavonoid biosynthetic pathways are coordinately activated for the production of flavonoids and phenolic phytoalexins such as sakuranetin and phenylamides. An in silico analysis of UV-induced O-methyltransferase and acyltransferase genes suggested that these genes may be implicated in sakuranetin and phenylamide synthesis, respectively. The transcriptomic analysis also showed up-regulation of both methylerythritol phosphate pathway and the diterpenoid phytoalexin biosynthetic genes in response to UV treatment. A functional gene network analysis of phytoalexin biosynthetic and UV-induced genes for signaling components and transcription factors using RiceNet suggested that regulatory networks comprising signal perceiving receptor kinases, G-proteins, signal transducing mitogen-activated protein kinases and calcium signaling components, and transcription factors control flavonoid and phytoalexin biosynthesis in rice leaves under UV-C stress conditions. |
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| Journal | Phytochemistry | |
| Country | South Korea | |
| Volume | 96 | |
| Pages | 57-71 | |
| Year | 2013 | |
| PubMed ID | 24035516 | |
| PubMed Central ID | - | |
| DOI | 10.1016/j.phytochem.2013.08.012 | |
| URL | - | |
| Relation | ||
| Gene | 2ODD17 2ODD2 4CL3 ASB2 ASMT1 CDPK24 CDPK8 CHI6 CHI7 CHS CM COMT20 COMTL2 CPS2 CRO1 CS CYP71Z7 CYP76M8 CYP99A2 CYP99A3 DFR4 DFR5 DNRL1 DXR DXS3 GDI4 GRY340 HSFB2B IGPS MAPK4 MCS MPK6 MRLK26 MRLK48 MSRMK2 NTMC2T6.2 PAL PAL1 PKS12 PKS2 ROMT9 SK1 SK2 TDC1 TDD1 TSA WRKY46 _ _ _ _ _ _ _ _ _ _ _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
