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著者 Yi N., Oh S.J., Kim Y.S., Jang H.J., Park S.H., Jeong J.S., Song S.I., Choi Y.D., Kim J.K.
タイトル Analysis of the Wsi18, a stress-inducible promoter that is active in the whole grain of transgenic rice
Abstract:
There is currently a shortage of efficient promoters for stress-inducible gene expression, especially in monocotyledonous crops. Here, we report analysis of the rice Wsi18 promoter, a member of the group 3 Lea family, in transgenic rice plants. The abundance of Wsi18 mRNA increased in leaf tissues within 2 h of exposure to NaCl or abscisic acid (ABA) and within 6 h of exposure to drought, but there was no transcript increase in response to low-temperature conditions. Wsi18 mRNA accumulated in the roots similarly to in the leaves, but at a faster rate. The promoter was linked to the GFP reporter gene, transformed into rice, and its activity was analyzed in transgenic plants at all stages of plant growth from calli, vegetative tissues, flowers, and to dry seeds, both before and after stress treatment. The activity of the promoter was significantly increased in the whole plant body, including flowers, on exposure of plants to stress conditions, with very low levels of basal activity in all tissues. Moreover, the promoter was found to be predominantly active in the whole grain, including endosperm, embryo, and aleurone layer during seed development. Together, we have identified and analyzed the Wsi18 promoter and found a previously undescribed characteristic-a stress-inducible property in the whole plant body with activity in the whole grain during seed development. 諱ｯ 2010 Springer Science+Business Media B.V.
掲載誌 Transgenic Res.
Country South Korea
刊数 20
ページ 153-163
発刊年 2011
PubMed ID 20490919
PubMed Central ID -
DOI -
URL -
関連情報
遺伝子 WSI18
INSD -
系統 野生イネ
コアコレクション 		-
突然変異系統(遺伝研 Collection) -
種子不稔系統 -
致死変異体系統 -
組織別発生段階
- 突然変異系統(遺伝子) 		-
栽培イネ品種(遺伝研 Collection) -
組織別発生段階 -
/rice/oryzabase