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Reference Detail
| Reference | ||
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| Author | Li D., Wang L., Wang M., Yun-Yuan Xu, Luo W., Ya-Ju Liu, Zhi-Hong Xu, Li J., Chong K. | |
| Title | Engineering OsBAK1 gene as a molecular tool to improve rice architecture for high yield. | |
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Abstract: Generating a new variety of plant with erect-leaf is a critical strategy to improve rice grain yield, as plants with this trait can be dense-planted. The erect-leaf is a significant morphological trait partially regulated by Brassinosteroids (BRs) in rice plants. So far, only a few genes can be used for molecular breeding in rice. Here, we identified OsBAK1 as a potential gene to alter rice architecture. Based on rice genome sequences, four closely related homologs of Arabidopsis BAK1 (AtBAK1) gene were amplified. Phylogenetic analysis and suppression of a weak Arabidopsis mutant bri1-5 indicated that OsBAK1 (Os08g0174700) is the closest relative of AtBAK1. Genetic, physiological, and biochemical analyses all suggest that the function of OsBAK1 is conserved with AtBAK1. Overexpression of a truncated intracellular domain of OsBAK1, but not the extracellular domain of OsBAK1, resulted in a dwarfed phenotype, similar to the rice BR-insensitive mutant plants. The expression of OsBAK1 changed important agricultural traits of rice such as plant height, leaf erectness, grain morphologic features, and disease resistance responses. Our results suggested that a new rice variety with erect-leaf and normal reproduction can be generated simply by suppressing the expression level of OsBAK1. Therefore, OsBAK1 is a potential molecular breeding tool for improving rice grain yield by modifying rice architecture. |
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| Journal | Plant Biotechnol. J. | |
| Country | China | |
| Volume | 7(8) | |
| Pages | 791-806 | |
| Year | 2009 | |
| PubMed ID | 19754838 | |
| PubMed Central ID | - | |
| DOI | 10.1111/j.1467-7652.2009.00444.x | |
| URL | - | |
| Relation | ||
| Gene | BZR1 D2 D61 SERK1 SG2 TMS10 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
