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著者 | Abiko M., Maeda H., Tamura K., Hara-Nishimura I., Okamoto T. | |
タイトル | Gene expression profiles in rice gametes and zygotes: identification of gamete-enriched genes and up- or down-regulated genes in zygotes after fertilization. | |
Abstract: In angiosperms, fertilization and subsequent zygotic development occur in embryo sacs deeply embedded in the ovaries; therefore, these processes are poorly elucidated. In this study, microarray-based transcriptome analyses were conducted on rice sperm cells, egg cells, and zygotes isolated from flowers to identify candidate genes involved in gametic and/or early zygotic development. Cell type-specific transcriptomes were obtained, and up- or down-regulated genes in zygotes after fertilization were identified, in addition to genes enriched in male and female gametes. A total of 325 putatively up-regulated and 94 putatively down-regulated genes in zygotes were obtained. Interestingly, several genes encoding homeobox proteins or transcription factors were identified as highly up-regulated genes after fertilization, and the gene ontology for up-regulated genes was highly enriched in functions related to chromatin/DNA organization and assembly. Because a gene encoding methyltransferase 1 was identified as a highly up-regulated gene in zygotes after fertilization, the effect of an inhibitor of this enzyme on zygote development was monitored. The inhibitor appeared partially to affect polarity or division asymmetry in rice zygotes, but it did not block normal embryo generation. |
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掲載誌 | J. Exp. Bot. | |
Country | Japan | |
刊数 | 64(7) | |
ページ | 1927-40 | |
発刊年 | 2013 | |
PubMed ID | 23570690 | |
PubMed Central ID | 3638821 | |
DOI | 10.1093/jxb/ert054 | |
URL | - | |
関連情報 | ||
遺伝子 | AGP3 AHA8 BEL BZIP34 CBSCLC1 CCH CCR3 CHR735 CRT3 FEB1 GH9B6 MATE34 MET1B MGD3 MSRMK2 NRFG4 OSCA1.3 RAD21-3 RAP1A RBOHB RPA1 RPP17 SUMO3 TIP4;1 WOX5 WRKY19 WRKY42 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ | |
INSD | - | |
系統 |
野生イネ コアコレクション |
- |
突然変異系統(遺伝研 Collection) | - | |
種子不稔系統 | - | |
致死変異体系統 | - | |
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
栽培イネ品種(遺伝研 Collection) | - | |
組織別発生段階 | - |