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著者 | Kim Y.J., Yeu S.Y., Park B.S., Koh H.J., Song J.T., Seo H.S. | |
タイトル | Protein disulfide isomerase-like protein 1-1 controls endosperm development through regulation of the amount and composition of seed proteins in rice. | |
Abstract: Protein disulfide isomerase (PDI) is a chaperone protein involved in oxidative protein folding by acting as a catalyst and assisting folding in the endoplasmic reticulum (ER). A genome database search showed that rice contains 19 PDI-like genes. However, their functions are not clearly identified. This paper shows possible functions of rice PDI-like protein 1-1 (PDIL1-1) during seed development. Seeds of the T-DNA insertion PDIL1-1 mutant, PDIL1-1Delta, identified by genomic DNA PCR and western blot analysis, display a chalky phenotype and a thick aleurone layer. Protein content per seed was significantly lower and free sugar content higher in PDIL1-1Delta mutant seeds than in the wild type. Proteomic analysis of PDIL1-1Delta mutant seeds showed that PDIL1-1 is post-translationally regulated, and its loss causes accumulation of many types of seed proteins including glucose/starch metabolism- and ROS (reactive oxygen species) scavenging-related proteins. In addition, PDIL1-1 strongly interacts with the cysteine protease OsCP1. Our data indicate that the opaque phenotype of PDIL1-1Delta mutant seeds results from production of irregular starch granules and protein body through loss of regulatory activity for various proteins involved in the synthesis of seed components. |
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掲載誌 | PLoS ONE | |
Country | South Korea | |
刊数 | 7(9) | |
ページ | e44493 | |
発刊年 | 2012 | |
PubMed ID | 22970232 | |
PubMed Central ID | 3435311 | |
DOI | 10.1371/journal.pone.0044493 | |
URL | - | |
関連情報 | ||
遺伝子 | CP1 PDIL1-1 | |
INSD | - | |
系統 |
野生イネ コアコレクション |
- |
突然変異系統(遺伝研 Collection) | - | |
種子不稔系統 | - | |
致死変異体系統 | - | |
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
栽培イネ品種(遺伝研 Collection) | - | |
組織別発生段階 | - |