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Reference
Author		Sun Y., Lin Z., Reinders A., Ward J.M.
Title		Functionally Important Amino Acids in Rice sucrose transporter OsSUT1.
Abstract: Six conserved, charged amino acids within membrane spans in rice sucrose transporter OsSUT1 were identified using a three-dimensional structural model based on the crystal structures of three major facilitator superfamily (MFS) proteins: LacY, GlpT, and EmrD. These positions in OsSUT1 were selected for mutagenesis and biochemical assays. Among the six mutants, D177N completely lost transport function, D331N retained only a small fraction of sucrose uptake activity (2.3% of that of the wild type), and R335H and E336Q also displayed a substantial decrease in transport activity. D329N functioned as well as wild-type OsSUT1. R188K did not transport sucrose but showed a H(+) leak that was inhibited by sucrose, indicating that R188K had uncoupled sucrose and H(+) translocation. This demonstrates that charged amino acids within membrane spans are important for the transport mechanism of OsSUT1 as they are in lactose permease.
Journal		Biochemistry
Country		USA
Volume		51(15)
Pages		3284-91
Year		2012
PubMed ID		22458969
PubMed Central ID		-
DOI		-
URL		-
Relation
Gene		SUT1
INSD		-
Strain	Wild Core Collection	-
	Induced Mutation Lines(NIG Collection)	-
	Sterile Seed Strain	-
	Lethal Embryo Mutantion Strain	-
	Stages in Each Organ - Muant Lines (Gene)	-
	Cultivated Varieties(NIG Collection)	-
Stages in Each Organ		-