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Reference Detail
| Reference | ||
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| Author | Nakamura Y., Ono M., Utsumi C., Steup M. | |
| Title | Functional Interaction between Plastidial Starch Phosphorylase and Starch Branching Enzymes from Rice during the Synthesis of Branched Maltodextrins. | |
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Abstract: The present study established the way how plastidial alpha-glucan phosphorylase (Pho1) synthesizes the malto-dextrin (MD) which can be the primer for starch biosynthesis in rice endosperm. The synthesis of MD by Pho1 was markedly accelerated by branching enzyme (BE) isozymes although the greatest effect was exhibited by the presence of branching isozyme I (BEI) rather than by isozyme IIa (BEIIa) or isozyme IIb (BEIIb). The enhancement of the activity of Pho1 by BE was not merely due to the supply of non-reducing end with Pho1. At the same time Pho1 greatly enhanced the BE activity, possibly by generating a branched carbohydrate substrate which is used by BE with a higher affinity. The addition of isoamylase to the reaction mixture did not prevent the concerted action of Pho1 and BEI. Furthermore, in the product was, at least to some extent, the branched structure maintained. Based on these results we propose that the interaction between Pho1 and BE is not merely due to chain-elongating and chain-branching reactions, but it is based on a physically and catalytically synergistic manner by activating the mutual capacities each other, presumably forming a physical association of Pho1, BEI and branched MD. This close interaction might play a crucial role in the synthesis of branched MD and the branched MD can act as primers for the biosynthesis of amylopectin molecules. |
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| Journal | Plant Cell Physiol. | |
| Country | Japan | |
| Volume | 53(5) | |
| Pages | 869-78 | |
| Year | 2012 | |
| PubMed ID | 22414443 | |
| PubMed Central ID | - | |
| DOI | - | |
| URL | - | |
| Relation | ||
| Gene | PHO1 SBE1 SBE3 SBE4 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
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Stages in Each Organ - Muant Lines (Gene) |
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| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
