研究支援ツール - 文献 - 詳細
文献詳細
文献 | ||
---|---|---|
著者 | Li Y., Chen Y., Wu J., He C. | |
タイトル | Expression and functional analysis of OsRboh gene family in rice immune response | |
Abstract: The preliminary role of respiratory burst oxidase homolog (Rboh) in plant immune response is defined, but the exact function of OsRboh gene in rice immune response and its expression pattern is yet unclear. In order to clarify the role of OsRboh in rice immune response, we screened seven OsRboh genes from the latest rice genome annotation database. The result of tissue specific expression analysis demonstrated that OsRbohD was expressed only in spike and calli, and OsRbohE and OsRbohF were only expressed in calli. The rest of OsRboh genes were constitutively expressed in rice. In addition, the expression level of OsRboh gene family was analyzed in the rice leaves respectively treated with salicylic acid (SA), methyl jasmonic acid (MeJA) and Xanthomonas oryzae PV. oryzae (Xoo) PXO99 strain by Real-time PCR, and H2O2 content was also quantified by spectrophotometry after the three treatments. The result shows that the expression of OsRbohA, B, C and D was increased under the treatments of SA, the expression of OsRbohA, B, C and G was increased under the treatments of MeJA, and the expression of OsRbohA and OsRbohB was induced by Xoo PXO99 strain. However, the levels of expression and responsive times of these genes were different. Moreover, all three treatments led to H2O2 accumulation. These OsRboh genes have functional roles in rice native immune response. |
||
掲載誌 | Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology | |
Country | China | |
刊数 | 27 | |
ページ | 1574-1585 | |
発刊年 | 2011 | |
PubMed ID | 22393712 | |
PubMed Central ID | - | |
DOI | - | |
URL | - | |
関連情報 | ||
遺伝子 | RBOHA RBOHB RBOHC RBOHD RBOHE RBOHG RBOHI | |
INSD | - | |
系統 |
野生イネ コアコレクション |
- |
突然変異系統(遺伝研 Collection) | - | |
種子不稔系統 | - | |
致死変異体系統 | - | |
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
栽培イネ品種(遺伝研 Collection) | - | |
組織別発生段階 | - |