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| 文献 | ||
|---|---|---|
| 著者 | Ozawa K., Wakasa Y., Ogo Y., Matsuo K., Kawahigashi H., Takaiwa F. | |
| タイトル | Development of an efficient agrobacterium-mediated gene targeting system for rice and analysis of rice knockouts lacking granule-bound starch synthase (Waxy) and beta1,2-xylosyltransferase | |
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Abstract: We have developed a high-frequency method for Agrobacterium-mediated gene targeting by combining an efficient transformation system using rice suspension-cultured calli and a positive/negative selection system. Compared with the conventional transformation system using calli on solid medium, transformation using suspension-cultured calli resulted in a 5-to 10-fold increase in the number of resistant calli per weight of starting material after positive/negative selection. Homologous recombination occurred in about 1.5 of the positive/negative selected calli. To evaluate the efficacy of our method, we show in this report that knockout rice plants containing either a disrupted Waxy (granule-bound starch synthase) or a disrupted Xyl (beta1,2- xylosyltransferase) gene can be easily obtained by homologous recombination. Study of gene function using homologous recombination in higher plants can now be considered routine work as a direct result of this technical advance. |
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| 掲載誌 | Plant and Cell Physiology | |
| Country | Japan | |
| 刊数 | 53 | |
| ページ | 755-761 | |
| 発刊年 | 2012 | |
| PubMed ID | 22327484 | |
| PubMed Central ID | - | |
| DOI | - | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | RCN11 WX1 | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
