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Author Inoue H., Mizuno D., Takahashi M., Nakanishi H., Mori S., Nishizawa N.K
Title A Rice FRD3-Like (OsFRDL1) Gene Is Expressed in the Cells Involved in Long-Distance Transport
Abstract:
We identified four putative AtFRD3-like genes (OsFRDL) in the rice genome that exhibited 39.1 to 56.7% amino acid sequence similarities to Arabidopsis FRD3. Of these, we cloned three OsFRDL genes from a cDNA library prepared from iron-deficient rice roots : OsFRDL1, OsFRDL2, and OsFRDL3. OsFRDL1 was expressed weakly in Fe-sufficient roots, and slight expression was induced in the roots of Fe-deficient plants. OsFRDL2 was expressed constitutively in both roots and leaves, and Fe deficiency reduced its expression in leaves. OsFRDL3 was expressed in leaves, but not in roots ; Fe deficiency induced slight expression in leaves. An OsFRDL1-sGFP fusion protein was localized in the plasma membrane in onion epidermal cells. The promoter GUS analysis showed that OsFRDL1 was localized in the cells involved in long-distance transport, in both Fe-sufficient and Fe-deficient plants. Furthermore, OsFRDL1 expression was observed during the reproductive stage. These results suggest that OsFRDLl is a transporter that resides in the plasma membrane of cells involved in long-distant transport.
Journal Soil Sci. Plant Nutr.
Country Japan
Volume 50(7)
Pages 1133-1140
Year 2004
PubMed ID -
PubMed Central ID -
DOI -
URL -
Relation
Gene FRDL1 FRDL2 FRDL3
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase