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Author Du L., Jiao F., Chu J., Jin G., Chen M., WU P.
Title The two-component signal system in rice (Oryza sativa L.): a genome-wide study of cytokinin signal perception and transduction.
Abstract:
In this report we define the genes of two-component regulatory systems in rice through a comprehensive computational analysis of rice (Oryza sativa L.) genome sequence databases. Thirty-seven genes were identified, including 5 HKs (cytokinin-response histidine protein kinase) (OsHK1-4, OsHKL1), 5 HPs (histidine phosphotransfer proteins) (OsHP1-5), 15 type-A RRs (response regulators) (OsRR1-15), 7 type B RR genes (OsRR16-22), and 5 predicted pseudo-response regulators (OsPRR1-5). Protein motif organization, gene structure, phylogenetic analysis, chromosomal location, and comparative analysis between rice, maize, and Arabidopsis are described. Full-length cDNA clones of each gene were isolated from rice. Heterologous expression of each of the OsHKs in yeast mutants conferred histidine kinase function in a cytokinin-dependent manner. Nonconserved regions of individual cDNAs were used as probes in expression profiling experiments. This work provides a foundation for future functional dissection of the rice cytokinin two-component signaling pathway.
Journal Genomics
Country China
Volume 89(6)
Pages 697-707
Year 2007
PubMed ID 17408920
PubMed Central ID -
DOI 10.1016/j.ygeno.2007.02.001
URL -
Relation
Gene AHP1 AHP2 CKT1 CRL4 DLC1 HST1 PHP1 PHP2 PHP3 RR12 RR13 RR21 RR23 RR25 RR26 RR27 RR28 RR29 RR8
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase