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著者 | Takahashi S., Kimura S., Kaya H., Iizuka A., Wong H.L., Shimamoto K., Kuchitsu K. | |
タイトル | Reactive oxygen species production and activation mechanism of the rice NADPH oxidase OsRbohB. | |
Abstract: Reactive oxygen species (ROS) produced by plant NADPH oxidases (NOXes) are important in plant innate immunity. The Oryza sativa respiratory burst oxidase homologue B (OsRbohB) gene encodes a NOX the regulatory mechanisms of which are largely unknown. Here, we used a heterologous expression system to demonstrate that OsRbohB shows ROS-producing activity. Treatment with ionomycin, a Ca(2+) ionophore, and calyculin A, a protein phosphatase inhibitor, activated ROS-producing activity; it was thus OsRbohB activated by both Ca(2+) and protein phosphorylation. Mutation analyses revealed that not only the first EF-hand motif but also the upstream amino-terminal region were necessary for Ca(2+)-dependent activation, while these regions are not required for phosphorylation-induced ROS production. |
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掲載誌 | J. Biochem. | |
Country | Japan | |
刊数 | 152(1) | |
ページ | 37-43 | |
発刊年 | 2012 | |
PubMed ID | 22528669 | |
PubMed Central ID | - | |
DOI | - | |
URL | - | |
関連情報 | ||
遺伝子 | RBOHB | |
INSD | - | |
系統 |
野生イネ コアコレクション |
- |
突然変異系統(遺伝研 Collection) | - | |
種子不稔系統 | - | |
致死変異体系統 | - | |
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
栽培イネ品種(遺伝研 Collection) | - | |
組織別発生段階 | - |