Tools - Reference - Detail
Reference Detail
Reference | ||
---|---|---|
Author | Takahashi S., Kimura S., Kaya H., Iizuka A., Wong H.L., Shimamoto K., Kuchitsu K. | |
Title | Reactive oxygen species production and activation mechanism of the rice NADPH oxidase OsRbohB. | |
Abstract: Reactive oxygen species (ROS) produced by plant NADPH oxidases (NOXes) are important in plant innate immunity. The Oryza sativa respiratory burst oxidase homologue B (OsRbohB) gene encodes a NOX the regulatory mechanisms of which are largely unknown. Here, we used a heterologous expression system to demonstrate that OsRbohB shows ROS-producing activity. Treatment with ionomycin, a Ca(2+) ionophore, and calyculin A, a protein phosphatase inhibitor, activated ROS-producing activity; it was thus OsRbohB activated by both Ca(2+) and protein phosphorylation. Mutation analyses revealed that not only the first EF-hand motif but also the upstream amino-terminal region were necessary for Ca(2+)-dependent activation, while these regions are not required for phosphorylation-induced ROS production. |
||
Journal | J. Biochem. | |
Country | Japan | |
Volume | 152(1) | |
Pages | 37-43 | |
Year | 2012 | |
PubMed ID | 22528669 | |
PubMed Central ID | - | |
DOI | - | |
URL | - | |
Relation | ||
Gene | RBOHB | |
INSD | - | |
Strain | Wild Core Collection | - |
Induced Mutation Lines(NIG Collection) | - | |
Sterile Seed Strain | - | |
Lethal Embryo Mutantion Strain |
- | |
Stages in Each Organ - Muant Lines (Gene) |
- | |
Cultivated Varieties(NIG Collection) | - | |
Stages in Each Organ | - |