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Author		Takahashi S., Kimura S., Kaya H., Iizuka A., Wong H.L., Shimamoto K., Kuchitsu K.
Title		Reactive oxygen species production and activation mechanism of the rice NADPH oxidase OsRbohB.
Abstract: Reactive oxygen species (ROS) produced by plant NADPH oxidases (NOXes) are important in plant innate immunity. The Oryza sativa respiratory burst oxidase homologue B (OsRbohB) gene encodes a NOX the regulatory mechanisms of which are largely unknown. Here, we used a heterologous expression system to demonstrate that OsRbohB shows ROS-producing activity. Treatment with ionomycin, a Ca(2+) ionophore, and calyculin A, a protein phosphatase inhibitor, activated ROS-producing activity; it was thus OsRbohB activated by both Ca(2+) and protein phosphorylation. Mutation analyses revealed that not only the first EF-hand motif but also the upstream amino-terminal region were necessary for Ca(2+)-dependent activation, while these regions are not required for phosphorylation-induced ROS production.
Journal		J. Biochem.
Country		Japan
Volume		152(1)
Pages		37-43
Year		2012
PubMed ID		22528669
PubMed Central ID		-
DOI		-
URL		-
Relation
Gene		RBOHB
INSD		-
Strain	Wild Core Collection	-
	Induced Mutation Lines(NIG Collection)	-
	Sterile Seed Strain	-
	Lethal Embryo Mutantion Strain	-
	Stages in Each Organ - Muant Lines (Gene)	-
	Cultivated Varieties(NIG Collection)	-
Stages in Each Organ		-