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Reference Detail
| Reference | ||
|---|---|---|
| Author | Ardie S.W., Nishiuchi S., Liu S., Takano T. | |
| Title | Ectopic expression of the K+ channel beta subunits from Puccinellia tenuiflora (KPutB1) and rice (KOB1) alters K+ homeostasis of yeast and Arabidopsis. | |
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Abstract: In this study, we cloned a cDNA for the K+ channel β subunit from the halophyte Puccinellia tenuiflora and named it KPutB1. KPutB1 was preferentially expressed in the roots and was transiently induced by K+-starvation, salt stress, or the combination of both stresses. By yeast two-hybrid assay, we demonstrated that KPutB1 interacts with PutAKT1, α subunit of an AKT1-type K+ channel of P. tenuiflora. The functional relevance of this interaction on K+-nutrition was investigated by co-expression experiments in yeast under various ionic conditions, and K+ channel α and β subunit homologues from rice (OsAKT1 and KOB1, respectively) were included for comparison. Yeast co-expressing PutAKT1 and the β subunits (KPutB1 and KOB1) had better growth and higher K+-uptake ability than yeast expressing PutAKT1 alone. In contrast, yeast co-expressing the β subunits (KPutB1 and KOB1) with OsAKT1 had slower growth and lower K+ uptake than yeast expressing OsAKT1 alone. Arabidopsis plants over-expressing the K+ channel β subunit of P. tenuiflora or rice showed increased shoot K+ content and decreased root Na+ content under control, 75 mM NaCl, and K+-starvation stress conditions. These results suggest that ectopic expression of the K+ channel β subunit could alter K+ and Na+ homeostasis in plants. |
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| Journal | Mol. Biotechnol. | |
| Country | Japan | |
| Volume | 48(1) | |
| Pages | 76-86 | |
| Year | 2011 | |
| PubMed ID | 21108023 | |
| PubMed Central ID | - | |
| DOI | - | |
| URL | - | |
| Relation | ||
| Gene | AKT1 KOB1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
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Stages in Each Organ - Muant Lines (Gene) |
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| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
