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Author Tsai Y.C., Weir N.R., Hill K., Zhang W., Kim H.J., Shiu S.H., Schaller G.E., Kieber J.J.
Title Characterization of genes involved in cytokinin signaling and metabolism from rice.
Abstract:
Two-component signaling elements play important roles in plants, including a central role in cytokinin signaling. We characterized two-component elements from the monocot rice (Oryza sativa) using several complementary approaches. Phylogenetic analysis reveals relatively simple orthologous relationships among the histidine kinases in rice and Arabidopsis (Arabidopsis thaliana). In contrast, the histidine-containing phosphotransfer proteins (OsHPs) and response regulators (OsRRs) display a higher degree of lineage-specific expansion. The intracellular localizations of several OsHPs and OsRRs were examined in rice and generally found to correspond to the localizations of their dicot counterparts. The functionality of rice type-B OsRRs was tested in Arabidopsis; one from a clade composed of both monocot and dicot type-B OsRRs complemented an Arabidopsis type-B response regulator mutant, but a type-B OsRR from a monocot-specific subfamily generally did not. The expression of genes encoding two-component elements and proteins involved in cytokinin biosynthesis and degradation was analyzed in rice roots and shoots and in response to phytohormones. Nearly all type-A OsRRs and OsHK4 were up-regulated in response to cytokinin, but other cytokinin signaling elements were not appreciably affected. Furthermore, multiple cytokinin oxidase (OsCKX) genes were up-regulated by cytokinin. Abscisic acid treatment decreased the expression of several genes involved in cytokinin biosynthesis and degradation. Auxin affected the expression of a few genes; brassinosteroid and gibberellin had only modest effects. Our results support a shared role for two-component elements in mediating cytokinin signaling in monocots and dicots and reveal how phytohormones can impact cytokinin function through modulating gene expression.
Journal Plant Physiol.
Country
Volume 158(4)
Pages 1666-84
Year 2012
PubMed ID 22383541
PubMed Central ID 3320177
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Relation
Gene AHP1 AHP2 CKT1 CKX1 CKX10 CKX11 CKX3 CKX4 CKX5 CKX6 CKX7 CKX8 CKX9 CRL4 DLC1 EF1 GN1A HD2 HK3 HK4 HK5 HST1 IPT1 IPT10 IPT2 IPT3 IPT4 IPT5 IPT6 IPT7 IPT8 IPT9 LABA1 LOG1 LOGL1 LOGL10 LOGL2 LOGL3 LOGL4 LOGL5 LOGL7 LOGL8 LOGL9 PHP1 PHP2 PHP3 PRR59 PRR73 PRR95 RR1 RR10 RR11 RR12 RR13 RR2 RR21 RR23 RR25 RR26 RR27 RR28 RR29 RR3 RR31 RR33 RR4 RR5 RR6 RR7 RR8 RR9 TOC1 _ _ _ _ _ _ _ _
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Strain Wild Core Collection -
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Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase