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著者 Endo M., Shimizu T., Toki S.
タイトル Selection of transgenic rice plants using a herbicide tolerant form of the acetolactate synthase gene.
Abstract:
Acetolactate synthase (ALS) is an enzyme in the biosynthetic pathway for branched-chain amino acids, and bispyribac-sodium (BS), a pyrimidinyl carboxy herbicide, is a well-known inhibitor of ALS activity. However, it appears that a mutated form of rice ALS [OsmALS (W548L/S627I)] confers resistance to BS. We succeeded in using OsmALS with native OsALS promoter and terminator region for a selection marker of rice transformation. Because this selection marker cassette is originally from the rice endogenous genome, it can be expected to be publicly acceptable.
掲載誌 Methods Mol. Biol.
Country Japan
刊数 847
ページ 59-66
発刊年 2012
PubMed ID 22350999
PubMed Central ID -
DOI -
URL -
関連情報
遺伝子 ALS
INSD -
系統 野生イネ
コアコレクション
-
突然変異系統(遺伝研 Collection) -
種子不稔系統 -
致死変異体系統 -
組織別発生段階
- 突然変異系統(遺伝子)
-
栽培イネ品種(遺伝研 Collection) -
組織別発生段階 -
/rice/oryzabase