更新情報研究支援ツール - 文献 - 詳細
文献詳細
| 文献 | ||
|---|---|---|
| 著者 | Zang B. , Li H. , Li W. , Deng X.W. , Wang X. | |
| タイトル | Analysis of trehalose-6-phosphate synthase (TPS) gene family suggests the formation of TPS complexes in rice. | |
|
Abstract: Trehalose-6-phosphate (T6P), an intermediate in the trehalose biosynthesis pathway, is emerging as an important regulator of plant metabolism and development. T6P levels are potentially modulated by a group of trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) homologues. In this study, we have isolated 11 TPS genes encoding proteins with both TPS and TPP domains, from rice. Functional complement assays performed in yeast tps1 and tps2 mutants, revealed that only OsTPS1 encodes an active TPS enzyme and no OsTPS protein possesses TPP activity. By using a yeast two-hybrid analysis, a complicated interaction network occurred among OsTPS proteins, and the TPS domain might be essential for this interaction to occur. The interaction between OsTPS1 and OsTPS8 in vivo was confirmed by bimolecular fluorescence complementation and coimmunoprecipitation assays. Furthermore, our gel filtration assay showed that there may exist two forms of OsTPS1 (OsTPS1a and OsTPS1b) with different elution profiles in rice. OsTPS1b was particularly cofractionated with OsTPS5 and OsTPS8 in the 360kDa complex, while OsTPS1a was predominantly incorporated into the complexes larger than 360kDa. Collectively, these results suggest that OsTPS family members may form trehalose-6-phosphate synthase complexes and therefore potentially modify T6P levels to regulate plant development. |
||
| 掲載誌 | Plant Mol. Biol. | |
| Country | China | |
| 刊数 | 76(6) | |
| ページ | 507-22 | |
| 発刊年 | 2011 | |
| PubMed ID | 21598083 | |
| PubMed Central ID | - | |
| DOI | 10.1007/s11103-011-9781-1 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | TPS1 TPS10 TPS11 TPS2 TPS3 TPS4 TPS5 TPS6 TPS7 TPS8 TPS9 | |
| INSD | HM050434 HM050424 HM050425 HM050426 HM050427 HM050428 HM050429 HM050430 HM050431 HM050432 HM050433 | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
|
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
