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Author Nock,C.J., Waters,D.L., Edwards,M.A., Bowen,S.G., Rice,N., Cordeiro,G.M. and Henry,R.J.
Title Chloroplast genome sequences from total DNA for plant identification
Abstract:
Chloroplast DNA sequence data are a versatile tool for plant identification or barcoding and establishing genetic relationships among plant species. Different chloroplast loci have been utilized for use at close and distant evolutionary distances in plants, and no single locus has been identified that can distinguish between all plant species. Advances in DNA sequencing technology are providing new cost-effective options for genome comparisons on a much larger scale. Universal PCR amplification of chloroplast sequences or isolation of pure chloroplast fractions, however, are non-trivial. We now propose the analysis of chloroplast genome sequences from massively parallel sequencing (MPS) of total DNA as a simple and cost-effective option for plant barcoding, and analysis of plant relationships to guide gene discovery for biotechnology. We present chloroplast genome sequences of five grass species derived from MPS of total DNA. These data accurately established the phylogenetic relationships between the species, correcting an apparent error in the published rice sequence. The chloroplast genome may be the elusive single-locus DNA barcode for plants.
Journal Plant Biotechnol J.
Country Australia
Volume 9(3)
Pages 328-333
Year 2011
PubMed ID 20796245
PubMed Central ID -
DOI 10.1111/j.1467-7652.2010.00558.x
URL -
Relation
Gene 16S rRNA 4.5S rRNA 4.5S rRNA 5S rRNA 5S rRNA CLPP INFA NDHA NDHB NDHC NDHD NDHE NDHF NDHG NDHH NDHI NDHJ NDHK PETA PETD PSAA PSAB PSAC PSBA PSBC PSBD PSBE PSBF PSBH PSBI PSBK PSBL PSBM PSBN RBCL RPL16 RPL2 RPL22 RPL23 RPL23 RPL32 RPL33 RPL36 RPOA RPOB RPOC1 RPS11 RPS14 RPS15 RPS15 RPS18 RPS19 RPS19 RPS2 RPS3 RPS4 RPS7 RPS7 RPS8 YCF4 _ _ _ _ _ _ _ _ _ _ _ _ _ _
INSD GU592207 GU592208 GU592209
Strain Wild Core Collection -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase