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Reference Detail
| Reference | ||
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| Author | Wakasa Y,Yasuda H,Oono Y,Kawakatsu T,Hirose S,Takahashi H,Hayashi S,Yang L,Takaiwa F | |
| Title | Expression of ER quality control-related genes in response to changes in BiP1 levels in developing rice endosperm. | |
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Abstract: Binding protein (BiP) is the key chaperone involved in folding of secretory proteins such as seed storage proteins in the ER lumen. To obtain functional information about BiP1, a gene that is predominantly expressed during rice seed maturation, we generated several transgenic rice plants in which various levels of BiP1 protein accumulated in an endosperm-specific manner. Severe suppression (BiP1 KD) or significant over-expression (BiP1 OEmax) of BiP1 not only altered seed phenotype and the intracellular structure of endosperm cells, but also reduced seed storage protein content, starch accumulation and grain weight. Microarray and RT-PCR analyses indicated that expression of many chaperone and co-chaperone genes was induced in transgenic plants, with more prominent expression in the BiP1 KD line than in the BiP1 OEmax line. Transcriptional induction of most chaperones was observed in calli treated with dithiothreitol or tunicamycin, treatments that trigger ER stress, indicating that induction of the chaperone genes in transgenic rice was caused by an ER stress response. In transient assays using rice protoplasts, the ortholog (Os06g0622700) of the AtbZIP60 transcription factor was shown to be involved in activation of some chaperone genes. Slight increases in the BiP1 level compared with wild-type, accompanied by increased levels of calnexin and protein disulfide isomerase-like proteins, resulted in significant enhancement of seed storage protein content, without any change in intracellular structure or seed phenotype. Judicious modification of BiP1 levels in transgenic rice can provide suitable conditions for the production of secretory proteins by alleviating ER stress. |
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| Journal | Plant J. | |
| Country | Japan | |
| Volume | 65(5) | |
| Pages | 675-89 | |
| Year | 2010 | |
| PubMed ID | 21223397 | |
| PubMed Central ID | - | |
| DOI | 10.1111/j.1365-313X.2010.04453.x | |
| URL | - | |
| Relation | ||
| Gene | BIP1 BIP2 BIP3 BIP5 CNX CRT2 ERDJ3B FES1C GLB1 GLU7 GLUA1 GLUA2 GLUB1 GLUB1A GLUB4 GLUC GLUD1 HSR203J O3 PDIL1-1 PDIL1-4 PDIL2-3 _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
