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Reference Detail
| Reference | ||
|---|---|---|
| Author | Swaminathan S,Morrone D,Wang Q,Fulton DB,Peters RJ | |
| Title | CYP76M7 is an ent-cassadiene C11alpha-hydroxylase defining a second multifunctional diterpenoid biosynthetic gene cluster in rice. | |
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Abstract: Biosynthetic gene clusters are common in microbial organisms, but rare in plants, raising questions regarding the evolutionary forces that drive their assembly in multicellular eukaryotes. Here, we characterize the biochemical function of a rice (Oryza sativa) cytochrome P450 monooxygenase, CYP76M7, which seems to act in the production of antifungal phytocassanes and defines a second diterpenoid biosynthetic gene cluster in rice. This cluster is uniquely multifunctional, containing enzymatic genes involved in the production of two distinct sets of phytoalexins, the antifungal phytocassanes and antibacterial oryzalides/oryzadiones, with the corresponding genes being subject to distinct transcriptional regulation. The lack of uniform coregulation of the genes within this multifunctional cluster suggests that this was not a primary driving force in its assembly. However, the cluster is dedicated to specialized metabolism, as all genes in the cluster are involved in phytoalexin metabolism. We hypothesize that this dedication to specialized metabolism led to the assembly of the corresponding biosynthetic gene cluster. Consistent with this hypothesis, molecular phylogenetic comparison demonstrates that the two rice diterpenoid biosynthetic gene clusters have undergone independent elaboration to their present-day forms, indicating continued evolutionary pressure for coclustering of enzymatic genes encoding components of related biosynthetic pathways. |
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| Journal | Plant Cell | |
| Country | USA | |
| Volume | 21(10) | |
| Pages | 3315-25 | |
| Year | 2009 | |
| PubMed ID | 19825834 | |
| PubMed Central ID | - | |
| DOI | 10.1105/tpc.108.063677 | |
| URL | - | |
| Relation | ||
| Gene | CPS2 CPS4 CYP71B13 CYP71W1 CYP71Z1 CYP71Z2 CYP71Z3 CYP71Z4 CYP71Z5 CYP71Z6 CYP71Z7 CYP71Z8 CYP76C2 CYP76L1 CYP76M1 CYP76M10 CYP76M13 CYP76M14 CYP76M2 CYP76M5 CYP76M6 CYP76M7 CYP76M8 CYP76M9 CYP99A2 CYP99A3 D35 KS1 KS4 KS5 KS6 KS7 KS8 KSL10 KSL11 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
