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Reference Detail
| Reference | ||
|---|---|---|
| Author | Piao R,Jiang W,Ham TH,Choi MS,Qiao Y,Chu SH,Park JH,Woo MO,Jin Z,An G,Lee J,Koh HJ | |
| Title | Map-based cloning of the ERECT PANICLE 3 gene in rice. | |
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Abstract: Panicle architecture in rice can have a strong influence on yield. Using N-methyl-N-nitrosourea mutagenesis, we isolated an erect panicle mutant, Hep, from Hwasunchalbyeo, a glutinous japonica rice cultivar. Genetic analysis revealed that the erect panicle phenotype was controlled by a single recessive mutation designated erect panicle 3 (ep3). Genetic mapping revealed that the ep3 mutation was located on the short arm of chromosome 2 in a 0.1 cM region delimited by the STS markers STS5803-5 and STS5803-7. The ep3 locus corresponded to 46.8 kb region and contained six candidate genes. Comparison of the DNA sequences of the candidate genes from wild-type and erect panicle plants revealed a single base-pair change in the second exon of LOC_Os02g15950, which is predicted to result in a nonsense mutation. LOC_Os02g15950 encodes a putative F-box protein containing 515 amino acids and is expressed throughout the plant during all growth stages. A line carrying a T-DNA insertion in LOC_ Os02g15950 was obtained and shown to have the same phenotype as the ep3 mutant, thus confirming the identification of LOC_Os02g15950 as the ERECT PANICLE 3 (EP3) gene. The ep3 mutation causes a significant increase in the number of small vascular bundles as well as the thickness of parenchyma in the peduncle, which results in the erect panicle phenotype. |
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| Journal | Theor. Appl. Genet. | |
| Country | South Korea | |
| Volume | 119(8) | |
| Pages | 1497-506 | |
| Year | 2009 | |
| PubMed ID | 19756471 | |
| PubMed Central ID | - | |
| DOI | 10.1007/s00122-009-1151-x | |
| URL | - | |
| Relation | ||
| Gene | EP3 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
